Reference number
ISO 3594:1976
© ISO 2024
ISO 3594:1976
Milk fat — Detection of vegetable fat by gas-liquid chromatography of sterols (Reference method)
Edition 1
1976-12
Withdrawn
Withdrawn (Edition 1, 1976)

Abstract

The method consists in preparing of sterol digitonides and dissolving in a mixture of formamide and dimethylformamide, extraction of the liberated sterols with pentane, separation of the sterols using a suitable gas chromatograph. If, on the chromatogram, a peak with the retention time of beta-sitosterol is obtained, the presence of vegetable fat in the sample is demonstrated. Peaks of other phytosterols may support this conclusion.

General information

  • Status
     : Withdrawn
    Publication date
     : 1976-12
    Stage
    : Withdrawal of International Standard [95.99]
  • Edition
     : 1
    Number of pages
     : 5
  • Technical Committee :
    ISO/TC 34/SC 5
    ICS :
    67.100.10 
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